PT  - JOURNAL ARTICLE
AU  - Tomohiro Sugiyama
AU  - Moriya Iwaizumi
AU  - Terumi Taniguchi
AU  - Satoshi Suzuki
AU  - Shinya Tani
AU  - Mihoko Yamade
AU  - Yasushi Hamaya
AU  - Satoshi Osawa
AU  - Takahisa Furuta
AU  - Hiroaki Miyajima
AU  - Tsutomu Ohta
AU  - Satoshi Baba
AU  - Haruhiko Sugimura
AU  - Masato Maekawa
AU  - Ken Sugimoto
TI  - Microsatellite frameshift variants in <em>SGO1</em> of gastric cancer are not always associated with MSI status
AID  - 10.1136/jclinpath-2020-206934
DP  - 2021 Jun 01
TA  - Journal of Clinical Pathology
PG  - 386--390
VI  - 74
IP  - 6
4099  - http://jcp.bmj.com/content/74/6/386.short
4100  - http://jcp.bmj.com/content/74/6/386.full
SO  - J Clin Pathol2021 Jun 01; 74
AB  - Aims Although frameshift variants in the microsatellite area of shugoshin 1 (SGO1) have been reported in the context of microsatellite instability-high (MSI-H)/deficient mismatch repair gastrointestinal cancer, most have been evaluated only in early stage I–III patients, and only two of its five microsatellite regions have been evaluated. Therefore, we investigated the frequency and MSI status of microsatellite frameshift variants in gastric cancer cases, including stage IV.Methods In a total of 55 cases, 30 gastric cancer resection and 25 non-resection cases, DNA was extracted from both tumour and normal parts and PCR was performed. The variant was confirmed by TA cloning, and MSI was evaluated using GeneMapper software.Results A frameshift variant of c.973delA was observed in 16 of the 45 evaluable cases. Its frequency was 35.6%. Of the 25 cases that could be assessed for MSI status, two cases of MSI-H were associated with the c.973delA SGO1 variant. However, c.973delA SGO1 variant was also observed in four cases of microsatellite stable.Conclusion Our study shows that SGO1 frameshift variants are not always associated with MSI status.All data relevant to the study are included in the article.