RT Journal Article
SR Electronic
T1 Variability in haemoglobin concentration by measurement tool and blood source: an analysis from seven countries
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 657
OP 663
DO 10.1136/jclinpath-2020-206717
VO 74
IS 10
A1 Aviva I Rappaport
A1 Crystal D Karakochuk
A1 Sonja Y Hess
A1 Ralph D Whitehead, Jr.
A1 Sorrel M L Namaste
A1 Omar Dary
A1 Megan E Parker
A1 Lynnette M Neufeld
A1 Leila M Larson
A1 Sam Newton
A1 Rita Wegmuller
A1 Denish Moorthy
YR 2021
UL http://jcp.bmj.com/content/74/10/657.abstract
AB Objective We explore factors such as the blood sampling site (capillary vs venous), the equipment (HemoCue vs automated haematology analyser) and the model of the HemoCue device (201+ vs 301) that may impact haemoglobin measurements in capillary and venous blood.Methods Eleven studies were identified, and bias, concordance and measures of diagnostic performance were assessed within each study.Findings Our analysis included 11 studies from seven countries (Cambodia, India, The Gambia, Ghana, Laos, Rwanda and USA). Samples came from children, men, non-pregnant women and pregnant women. Mean bias ranged from −8.7 to 2.5 g/L in Cambodian women, 6.2 g/L in Laotian children, 2.4 g/L in Ghanaian women, 0.8 g/L in Gambian children 6–23 months and 1.4 g/L in Rwandan children 6–59 months when comparing capillary blood on a HemoCue to venous blood on a haematology analyser. Bias was 8.3 g/L in Indian non-pregnant women and 2.6 g/L in Laotian children and women and 1.5 g/L in the US population when comparing capillary to venous blood using a HemoCue. For venous blood measured on the HemoCue compared with the automated haematology analyser, bias was 5.3 g/L in Gambian pregnant women 18–45 years and 11.3 g/L in Laotian children 6–59 months.Conclusion Our analysis found large variability in haemoglobin concentration measured on capillary or venous blood and using HemoCue Hb 201+ or Hb 301 or automated haematology analyser. We cannot ascertain whether the variation is due to differences in the equipment, differences in capillary and venous blood, or factors affecting blood collection techniques.Data are available upon reasonable request. Data provided for this pooled analysis were received from each co-author as deidentified participant data.