RT Journal Article
SR Electronic
T1 Low expression of DDX5 is associated with poor prognosis in patients with pancreatic ductal adenocarcinoma
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP 741
OP 745
DO 10.1136/jclinpath-2020-207002
VO 74
IS 11
A1 Morimachi, Masashi
A1 Hirabayashi, Kenichi
A1 Takanashi, Yumi
A1 Kawanishi, Aya
A1 Saika, Tsubasa
A1 Ueyama, Yumiko
A1 Nakagohri, Toshio
A1 Nakamura, Naoya
A1 Suzuki, Hidekazu
A1 Kagawa, Tatehiro
YR 2021
UL http://jcp.bmj.com/content/74/11/741.abstract
AB Aims Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal malignancies. Hence, there is a need for new markers and treatment strategies. P68/DEAD box protein 5 (DDX5) is an ATP-dependent RNA helicase of the DEAD box protein family. It is a prognostic marker for several cancers. In this study, we aimed to evaluate the expression and clinical relevance of DDX5 in PDAC.Methods DDX5 expression in tissue microarray blocks containing 230 PDAC samples was examined using immunohistochemical analysis. DDX5 expression was considered high when more than 50% of the cells were stained and low when less than 50% of the cells were stained. We investigated the association between DDX5 expression and clinicopathological parameters, including patient survival.Results The nuclei of normal pancreatic ducts, normal acinar cells and PDAC cells were stained positive for DDX5 although the intensity and distribution of DDX5 expression varied. Islet cells showed strong and diffuse staining of DDX5. DDX5 expression was low and high in 148 (64.3%) and 82 cases (35.7%), respectively. Low DDX5 expression was significantly associated with an advanced pT factor (pT2–pT3: tumour size,>20 mm), lymphatic involvement, advanced tumour-node-metastasis (TNM) stage (stages IIB, III, and IV), and venous involvement. In addition, the multivariate analysis revealed that DDX5 expression is an independent prognostic factor for PDAC.Conclusion These results suggest that DDX5 plays an important role in tumour invasiveness and PDAC prognosis.All data relevant to the study are included in the article.