PT  - JOURNAL ARTICLE
AU  - Jack K Tung
AU  - Kelly A Devereaux
AU  - Archana Lal Erdmann
AU  - Iris Schrijver
AU  - James Zehnder
AU  - Carlos J Suarez
TI  - Potential pitfalls in multiplex PCR-based next-generation sequencing: a case-based report
AID  - 10.1136/jclinpath-2021-208105
DP  - 2022 Feb 09
TA  - Journal of Clinical Pathology
PG  - jclinpath-2021-208105
4099  - http://jcp.bmj.com/content/early/2022/02/09/jclinpath-2021-208105.short
4100  - http://jcp.bmj.com/content/early/2022/02/09/jclinpath-2021-208105.full
AB  - Amplicon-based next-generation sequencing (NGS) assays employ highly sensitive, rapid, and cost-effective methods to detect clinically actionable mutations for the diagnosis, prognosis, and treatment of patients with cancer. However, recognition of certain limitations inherent to amplicon-based NGS assays is crucial for the correct interpretation and reporting of variants in the clinical setting. In this report, we illustrate three different potential pitfalls related to amplicon-based NGS assays based on our institutional experience and highlight how the risk of such events can be minimised.