RT Journal Article SR Electronic T1 Potential pitfalls in multiplex PCR-based next-generation sequencing: a case-based report JF Journal of Clinical Pathology JO J Clin Pathol FD BMJ Publishing Group Ltd and Association of Clinical Pathologists SP jclinpath-2021-208105 DO 10.1136/jclinpath-2021-208105 A1 Jack K Tung A1 Kelly A Devereaux A1 Archana Lal Erdmann A1 Iris Schrijver A1 James Zehnder A1 Carlos J Suarez YR 2022 UL http://jcp.bmj.com/content/early/2022/02/09/jclinpath-2021-208105.abstract AB Amplicon-based next-generation sequencing (NGS) assays employ highly sensitive, rapid, and cost-effective methods to detect clinically actionable mutations for the diagnosis, prognosis, and treatment of patients with cancer. However, recognition of certain limitations inherent to amplicon-based NGS assays is crucial for the correct interpretation and reporting of variants in the clinical setting. In this report, we illustrate three different potential pitfalls related to amplicon-based NGS assays based on our institutional experience and highlight how the risk of such events can be minimised.