RT Journal Article
SR Electronic
T1 Microsatellite instability evaluation of patients with solid tumour: routine practice insight from a large series of Italian referral centre
JF Journal of Clinical Pathology
JO J Clin Pathol
FD BMJ Publishing Group Ltd and Association of Clinical Pathologists
SP jclinpath-2022-208203
DO 10.1136/jclinpath-2022-208203
A1 Gianluca Russo
A1 Francesco Pepe
A1 Pasquale Pisapia
A1 Lucia Palumbo
A1 Mariantonia Nacchio
A1 Elena Vigliar
A1 Pierlorenzo Pallante
A1 Paola Parente
A1 Matteo Fassan
A1 Paolo Graziano
A1 Claudio Bellevicine
A1 Giancarlo Troncone
A1 Umberto Malapelle
A1 Antonino Iaccarino
YR 2022
UL http://jcp.bmj.com/content/early/2022/05/11/jclinpath-2022-208203.abstract
AB DNA mismatch repair complex is involved in the maintenance of DNA stability. In the recent years, a plethora of technical approaches for microsatellite instability (MSI) analysis emerged. Here, we review the results of our MSI status evaluation by adopting a customised workflow on microfluidic system obtained in 4 years of diagnostic routine practice. Data from MSI status were retrieved from our institutional archive covering the period from January 2017 to December 2021. Microfluidic analysis was carried out on microfluidic platform. Results were inspected with a proprietary software. Overall, microsatellite stability (MSS) and MSI-high (MSI-H) profile was detected in n=423/458 (92.36%) and n=35/458 (7.64%) patients with metastatic CRC (mCRC), respectively. In addition, n=78/86 (90.70%) and n=8/86 (9.30%) patients without CRC showed an MSS and MSI-H profile. This review highlights the suitability of microfluidic approach in patients with cancer for MSI testing.