TY - JOUR T1 - Technical note on the exploration of COVID-19 in autopsy material JF - Journal of Clinical Pathology JO - J Clin Pathol SP - 418 LP - 423 DO - 10.1136/jcp-2022-208525 VL - 76 IS - 6 AU - Matthew Phillip Humphries AU - Victoria Bingham AU - Fatima Abdullah Sidi AU - Stephanie Craig AU - Beatrize Lara AU - Hesham El-daly AU - Nicole O'Doherty AU - Perry Maxwell AU - Claire Lewis AU - Stephen McQuaid AU - James Lyness AU - Jacqueline James AU - David R J Snead AU - Manuel Salto-Tellez Y1 - 2023/06/01 UR - http://jcp.bmj.com/content/76/6/418.abstract N2 - Interrogation of immune response in autopsy material from patients with SARS-CoV-2 is potentially significant. We aim to describe a validated protocol for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease using multiplex immunofluorescence (mIF).The application of validated assays for the detection of SARS-CoV-2 in tissues, originally developed in our laboratory in the context of oncology, was used to map the topography and complexity of the adaptive immune response at protein and mRNA levels.SARS-CoV-2 is detectable in situ by protein or mRNA, with a sensitivity that could be in part related to disease stage. In formalin-fixed, paraffin-embedded pneumonia material, multiplex immunofluorescent panels are robust, reliable and quantifiable and can detect topographic variations in inflammation related to pathological processes.Clinical autopsies have relevance in understanding diseases of unknown/complex pathophysiology. In particular, autopsy materials are suitable for the detection of SARS-CoV-2 and for the topographic description of the complex tissue-based immune response using mIF. ER -