Effect of zinc fixation on antigen preservation
| Staining score | ||||||||
|---|---|---|---|---|---|---|---|---|
| Fixation | Dehydration | MHC II | CD11c | B220 | Mac-1 | CD3 | CD4 | CD8 |
| Acetone: one bath of 100% acetone for six hours. | ||||||||
| Ethanol: graded series of 30%, 50%, 70%, 90%, and 100% of ethanol, 30 minutes in each bath. | ||||||||
| –, Negative; +, weakly positive; ++, positive; +++, strongly positive. | ||||||||
| MHC, major histocompatibility complex. | ||||||||
| Acetone | +++ | +++ | +++ | +++ | – | – | – | |
| Zinc fixative (3 days) | Acetone | +++ | +++ | +++ | +++ | ++ | ++ | ++ |
| Zinc fixative (3 days) | Ethanol | +++ | +++ | +++ | +++ | ++ | ++ | ++ |