Table 1

Cellular distribution pattern of Chlamydia pneumoniae components at different times after infection in the lungs of mice infected intranasally with C pneumoniae

C pneumoniae antigens
Type of cellsDays post infectionC pneumoniae DNA and 16S rRNAMembrane proteinLipopoly-saccharideHeat shock protein 60Staining pattern
The results are the combined results of three mice at two days, three mice at seven days, and four mice at 21 days after infection. All mice at the same time point after infection showed a similar staining pattern.
Chlamydia pneumoniae DNA and 16S rRNA were determined by in situ hybridisation; the staining pattern was always inclusion-like.
Chlamydia pneumoniae antigens were determined by immunocytochemistry.
Number of positive cells graded: –, none; ±, <5/section; +, 5–25/section; ++, 26–100/section; +++, >100/section; ND, not done.
Staining patern: G, granular; I, inclusion-like; NA, not applicable.
Bronchus and alveolar epithelium2+++++++I
7ND+++I
21±NA
Alveolar macrophages2+++++++G
7ND++++++G
21++±G
Infiltrate cells2++±++I and G
7ND+++++I and G
21±+/–+G
Macrophages in bronchus associated lymphoid tissue2NA
7ND±±±G
21±+++G