The main technical parameters used by participants and the organising laboratory of UK NEQAS-ICC in the immunohistochemical demonstration of oestrogen receptors (ER) and progesterone receptors (PR)
| Oestrogen receptor: run 41 | Progesterone receptor: run 39 | |||||
|---|---|---|---|---|---|---|
| Ag retrieval | 10 Ab clone | Detection | Ag retrieval | 10 Ab clone | Detection | |
| Numbers shown in parenthesis relate to the number of laboratories using each antibody or system for run 41 (ER ∼ 178 returns) and run 39 (PR and ER, ∼ 176 returns) | ||||||
| In the runs for which PR was requested, laboratories not stocking an antibody to PR were requested to demonstrate ER. | ||||||
| 10, primary; ABC, avidin biotin complex; Ab, antibody; Ag, antigen; MW, microwave oven pretreatment; LSAB, labelled St ABC; “Other”, antibodies or systems each used by less than four participants, or incomplete data; PC, pressure cooker pretreatment; St ABC, streptomyces ABC. | ||||||
| Clinically validated labs (6) | MW (4) | 1D5 (5) | LSAB (1) | MW (3) | 1A6 (3) | LSAB (1) |
| PC (2) | 6F11 (1) | St ABC (3) | PC (2) | KD68 (3) | St ABC (4) | |
| ABC (2) | None (1) | ABC (1) | ||||
| All UK NEQAS-ICC participating labs | MW (96) | 1D5 (131) | St ABC (61) | MW (106) | 1A6 (76) | St ABC (64) |
| PC (60) | 6F11 (41) | ABC(50) | PC (55) | 1D5 (52) | ABC (43) | |
| PC in MW (18) | Other (6) | LSAB (23) | PC in MW (5) | 6F11 (10) | LSAB (41) | |
| Other (4) | Other (44) | Other (10) | Other (38) | Other (28) | ||
| UK NEQAS organising lab | PC | 1D5 | St ABC | PC | 1A6 | St ABC |