Primers and probes used for detection of human papillomavirus (HPV) DNAs by the polymerase chain reaction (PCR) and Southern blot analysis
| Primer/Probe | Product (bp) | |
|---|---|---|
| HPV-16 E7 | ||
| Sense | 5`-CCAGAGACAACTGATCTCTAC-3` | 171 |
| Antisense | 5`-GTGTGTGCTTTGTACGCACAAC-3` | |
| Probe | 5`-TAACCTTTTGTTGCAAGTGTGACTCTACGCTTCG-3` | |
| HPV-18 E7 | ||
| Sense | 5`-GAGCCGAACCACAACGTCAC-3` | 152 |
| Antisense | 5`-GGATGCACACCACGGACACA-3` | |
| Probe | 5`-TCCAGCAGCTGTTTCTGAACACCCTG-3` | |
| HPV-6 E6 | ||
| Sense | 5`-GCTGGATATGCAACAACAGTTG-3` | 189 |
| Antisense | 5`-CATGCATGTTGTCCAGCAGTG-3` | |
| Probe | 5`-GCTACCTGTGTCACAAACCG-3` | |
| HPV-11 E6 | ||
| Sense | 5`-GCAGCGTGTGCCTGTTGCTTA-3` | 230 |
| Antisense | 5`-AAGCAACGACCCTTCCACTGG-3` | |
| Probe | 5`-CCTGTGTCACAAGCCGTTGTG-3` | |
| HPV-16 E6 | ||
| Sense | 5`-GATGGGAATCCATATGCTGTA-3` | 240 |
| Antisense | 5`-TCGACCGGTCCACCGACCCCT-3` | |
| Probe | 5`-GCCACTGTGTCCTGAAGAAAAGC-3` | |
| HPV-18 E6 | ||
| Sense | 5`-CAGTATACCCCATGCTGCATGCC-3` | 160 |
| Antisense | 5`-TCGACCGGTCCACCGACCCCT-3` | |
| Probe | 5`-GCCACTGTGTCCTGAAGAAAAGC-3` | |