Table 2

Summary of comparative studies of nucleic acid amplification tests on clinical specimens

Overall valuesSmear positive specimensSmear negative specimens
StudyTests comparedNo of specimensSpecimen typesSensSpecPPVNPVSensSpecPPVNPVSensSpecPPVNPV
*Results after discrepant analysis and/or use of adapted gold standard.
†Results before discrepant analysis.
‡New version with shortened reaction times.
MAC, Mycobacterium avium complex; NPV, negative predictive value; PPV, positive predictive value; sens, sensitivity; spec, specificity.
Piersimoni and colleagues27AMTD327 (236)Respiratory and gastric aspirates95.9*98.9*94.0*99.2*100*100*100*100*87.5*98.8*82.3*99.2*
Amplicor85.4*99.6*97.9*97.1*97.0*100*100*99.6*66.8*99.6*93.3*97.4*
Della-Latta and Whittier28AMTD1385Respiratory97.1*99.5*100*100*92.9*99.5*
88.7†95.3†97.4†78.6†75.0†95.5†
Amplicor138096.7*100*97.4*100*95.9*100*
87.5†99.7†97.4†92.9†79.2†99.8†
Piersimoni and colleagues29AMTD II‡273Respiratory92.8*99.4*98.5*97.0*100*100*100*100*85.3*99.4*96.7*97.3*
LCx75.7†98.8†96.4†90.5†91.6†100†100†98.3†58.8†98.8†90.9†92.7†
AMTD II184Non-respiratory78.6*99.3*95.6*96.2*100*100*100*100*64.7*99.3*91.7*96.2*
LCx53.6†99.3†93.7†92.1†81.8†100†100†98.8†35.3†99.3†85.7†93.2†
Eing and colleagues30Cobas Amplicor1681Respiratory and non-respiratory66.3*99.7*94.4*97.7*87.5*99.4*56.5*99.7*90.7*97.9*
76.9†98.6†70.2†99.0†96.4†75.0†96.4†75.0†62.2†98.6†53.5†99.1†
In house IS6110 based PCR91.1*99.8*97.9*99.4*96.9*99.4*88.4*99.8*96.8*99.5*
90.8†97.6†62.7†99.6†100†25.0†90.3†100†83.8†97.6†46.2†99.3†
Gamboa and colleagues31Cobas Amplicor755 (450)Respiratory92.4*100*100*96.5*100*59.6*
Manual Amplicor90.8*100*100*95.8*100*51.0*
Ichiyama and colleagues32BDProbeTec-SDA TB530 (299)Sputum94.7*99.8*
Amplicor TB89.5*100*
BDProbeTec-SDA MAC82.8*98.3*
Amplicor MAC79.3*100*