Results on intraoperative assessment of SLNs
| Method | Ref. | N | Levels | Stains | Final histology | TP | TN | FP | FN | Acc. | Sens. | Spec. | PPV | NPV | FNR | FRR |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Levels refers to the levels sampled during intraoperative assessment. | ||||||||||||||||
| Stains refers to the stains used for intraoperative assessment. | ||||||||||||||||
| Final histology refers to the final histology that served as a basis for comparison. | ||||||||||||||||
| *On an SLN and **on a grossly negative SLN (and not patient) basis. | ||||||||||||||||
| Acc., accuracy (overall predictive value); DQ, Diff-Quik; FN, false negatives; FNR, false negative rate (false negatives/all positives); FP, false positives; FRR, false reassurance rate (false negatives/false and true negatives); FS, frozen section; HE, haematoxylin and eosin; IC, imprint cytology; IHC, immunohistochemistry to epithelial markers; MG, May-Giemsa; N, number of patients; NI: no information; NPV, negative predictive value; PPV, positive predictive value; RAL, rapid cytological stain RAL 555; Sens., sensitivity; Spec., specificity; TN, true negatives; TP, true positives. | ||||||||||||||||
| FS | 79 | 107 | 3 consecutive | HE | 3 HE | 32 | 57 | 0 | 18 | 83% | 64% | 100% | 100% | 76% | 36% | 24% |
| FS | 77 | 56 | NI | HE | HE | NI | NI | 0 | 3 | 95% | NI | NI | NI | NI | NI | NI |
| FS | 115 | 96 | 3 (each side) | HE | HE | 24 | 68 | 0 | 4 | 96% | 86% | 100% | 100% | 94% | 14% | 6% |
| FS | 116 | 47* | NI | HE | HE | 10 | 36 | 0 | 1 | 98% | 91% | 100% | 100% | 97% | 9% | 3% |
| FS | 117 | 54 | 2 | HE | HE more + IHC | 21 | 31 | 0 | 2 | 96% | 91% | 100% | 100% | 94% | 9% | 6% |
| 74* | 2 | HE | HE more + IHC | 27 | 43 | 0 | 4 | 95% | 87% | 100% | 100% | 91% | 13% | 9% | ||
| FS | 118 | 28 | 1 (2) | HE | IHC | 6 | 17 | 0 | 5 | 82% | 55% | 100% | 100% | 77% | 45% | 23% |
| FS | 119 | 62 | ≥ 1 | HE | HE + IHC same | 19 | 34 | 0 | 9 | 85% | 68% | 100% | 100% | 79% | 32% | 21% |
| IC + FS | 109, 120 | 278 | 1 | DQ | HE same level | 53 | 206 | 0 | 19 | 93% | 74% | 100% | 100% | 92% | 26% | 8% |
| 278 | 1 | DQ | HE more + IHC | 53 | 167 | 0 | 58 | 79% | 48% | 100% | 100% | 74% | 52% | 26% | ||
| IC + FS | 119 | 38 | ≥ 1 | MG + IHC/HE | HE + IHC same | 3 | 25 | 0 | 10 | 92% | 77% | 100% | 100% | 89% | 23% | 11% |
| IC | 117 | 45 | 2 | DQ | HE more + IHC | 14 | 23 | 0 | 8 | 82% | 64% | 100% | 100% | 74% | 36% | 26% |
| *59 | 2 | DQ | HE more + IHC | 16 | 33 | 0 | 10 | 83% | 62% | 100% | 100% | 77% | 38% | 23% | ||
| IC | 119 | 38 | 1 | MG + IHC | HE + IHC same | 6 | 25 | 0 | 7 | 82% | 46% | 100% | 100% | 78% | 54% | 22% |
| IC | 107 | 381** | 2 | DQ | HE more + IHC | 15 | 254 | 1 | 35 | 88% | 30% | 100% | 94% | 88% | 70% | 12% |
| IC | 121 | 124* | 1 | HE | HE same level | 22 | 101 | 0 | 1 | 99% | 96% | 100% | 100% | 99% | 5% | 1% |
| IC | 110 | 55 | 2 | HE | HE same level | 14 | 40 | 0 | 1 | 98% | 93% | 100% | 100% | 98% | 7% | 2% |
| IC | 122 | 25 | 1 | RAL | NI | 4 | 19 | 0 | 2 | 92% | 66% | 100% | 100% | 90% | 33% | 10% |
| IC | 123 | 161** | 2 | IHC | HE more + IHC | 30 | 126 | 0 | 5 | 97% | 86% | 100% | 100% | 96% | 14% | 4% |