Results of four cDNA microarrays with chondrosarcomas (CS)
| 1 | 2 | 3 | 4 | |
|---|---|---|---|---|
| *Non-normalised signal obtained by analysis with GenePix 3.0 package; “no flags” refers to the mean signal of only the reliable spots on the array. | ||||
| †Number of spots that were not detectable because of weak signal or bad signal to noise ratio. | ||||
| ‡Ratio of the Cy5 versus the Cy3 ratio, normalised according to the GenePix 3.0 program. | ||||
| Cy3, fluorescence at 532 nm; Cy5, fluorescence at 635 nm. | ||||
| Cy5 sample | OUMS cell line | Peripheral CS | Normal cartilage | Normal cartilage |
| Cy3 sample | SW1353 cell line | Central CS | Central CS | Peripheral CS |
| Mean signal/no flags: Cy5* | 3078/4026 | 1299/1947 | 907/1192 | 949/1377 |
| Mean background/no flags Cy5* | 499/486 | 575/560 | 627/5726 | 662/686 |
| Mean signal/no flags Cy3* | 3095/4077 | 1155/1842 | 440/613 | 489/817 |
| Mean background/no flags Cy3* | 512/527 | 402/405 | 344/340 | 267/274 |
| No. of flags† | 662 | 1150 | 1448 | 1452 |
| Range of ratios‡ | 49–0.067 | 46–0.053 | 22–0.061 | 27–0.021 |
| Cy5/Cy3 ratios >3 | 236 | 57 | 31 | 23 |
| Cy5/Cy3 ratios >2 | 421 | 130 | 93 | 109 |
| Cy3/Cy5 ratios >3 | 225 | 59 | 31 | 76 |
| Cy3/Cy5 ratios >2 | 438 | 155 | 65 | 125 |