Primers and probes used in fluorogenic probe assays
| Primer name | Sequence 5′ → 3′ |
|---|---|
| The table shows the primer and probe sequences used for the detection of human papillomavirus (HPV) transcripts and cellular telomerase reverse transcriptase (hTERT), telomerase RNA component (hTR), survivin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcripts. | |
| E6For | CAAACCGTTGTGTGATTTGTTAATTA |
| E6Rev | GCTTTTTGTCCAGATGTCTTTGC |
| E6Probe | TGTATTAACTGTCAAAAGCCACTGTGTCCTGAAGAA |
| E6*I | TACTGCGACGTGAGGTGTATTAAC |
| E6*II | TACTGCGACGTGAGATCATCA |
| E6*Common | CAGTTGTCTCTGGTTGCAAATC |
| E6*Probe | TATCTCCATGCATGATTACAGCTGGGTTTCTCTAC |
| E7For | ACCGGACAGAGCCCATTACA |
| E7Rev | GCCCATTAACAGGTCTTCCAAA |
| E7Probe | ATTGTAACCTTTTGTTGCAAGTGTGACTCTACGCTT |
| L1For | CGTTATGACATACATACATTCTATGAATTCC |
| L1Rev | TGGGTTACAAACCTATAAGTATCTTCTAGTGT |
| L1Probe | CTGGAATTTTGGTCTACAACCTCCCCCAG |
| GAPDHFor | GGGAAGGTGAAGGTCGGAGT |
| GAPDHRev | TGGAAGATGGTGATGGGATTTC |
| GAPDHProbe | ATTGACCTCAACTACATGGTTTACATGTTCCAATATGA |
| hTERTFor | CGGAAGAGTGTCTGGAGCAA |
| hTERTRev | CGACGTAGTCCATGTTCACAATC |
| hTERTProbe | CTTCAAGTGCTGTCTGATTCCAATGCTTTGC |
| hTRFor | GGTGGTGGCCATTTTTTGTC |
| hTRRev | CTAGAATGAACGGTGGAAGGC |
| hTRProbe | CGCGCTGTTTTTCTCGCTGACTTTC |
| SurvFor | GCGCTTTCCTTTCTGTCAAGA |
| SurvRev | GCACGGCGCACTTTCTT |
| SurvProbe | CCTCAAATTCTTTCTTCTTATTGTTGGTTTCCTTTGCC |