Reactivity of Centres for Disease Control (Atlanta, Georgia, USA) prototype sera in six detection systems for anti-Ro, anti-La, anti-Sm, and anti-U1-RNP
| Method | ||||||
|---|---|---|---|---|---|---|
| Prototype serum | CIE-IH | CIE-Com | ELISA-1 | ELISA-2 | ELISA-3 | WB-Com |
| Results are expressed as those specificities detected in each prototype serum. | ||||||
| ELISAs 1, 2, and 3 are from different manufacturers. | ||||||
| CIE-Com, countercurrent immunoelectrophoresis (commercial); CIE-IH, countercurrent immunoelectrophoresis (in house); ELISA, enzyme linked immunosorbent assay; RNP, ribonuclear protein; WB-Com, western blotting (commercial). | ||||||
| Anti-Ro | Ro | Ro | Ro | Negative | Ro | Negative |
| Anti-La | La | La | Ro + La | La | Ro + La | La |
| Anti-Sm | Sm | Sm | Sm/RNP | Sm/RNP | Sm/RNP | Sm |
| Anti-U1-RNP | RNP | RNP | RNP | RNP | RNP | RNP |