Transcript values detected in cell lines and cervical tumours
| HPV-16 transcript | Cellular transcript | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cells | E6 | E6*I | E6*II | E7 | L1 | GAPDH | hTERT | hTR | Survivin |
| DNA and RNA were extracted from cervical cancer cell lines containing HPV-16 (CaSki and SiHa) and biopsies from two patients with a cervical tumour containing HPV-16: a fast growing tumour from a 34 year old women (tumour 1) and a slow growing tumour from a women of 78 (tumour 2). | |||||||||
| Transcript measurements were performed by fluorogenic probe assay and are presented as copy number/cell. The average HPV-16 genome copy number/cell was 815 for CaSki cells, 3 for SiHa cells, 6 × 10−1 for the fast growing tumour, and 11 for the slow growing tumour. | |||||||||
| GAPDH, glyceraldehyde-3-phosphate dehydrogenase; hTERT, human telomerase reverse transcriptase; hTR, human telomerase RNA component. | |||||||||
| CaSki | 2 × 10−1 | Not detected | Not detected | 6.00 | 5 × 10−3 | 7.00 | 6 × 10−5 | 1.00 | 2 × 10−1 |
| SiHa | 1.00 | 1.00 | 4 × 10−2 | 9.00 | 2 × 10−3 | 126.00 | 3 × 10−3 | 2.00 | 3.00 |
| Tumour 1 | 2.00 | 2.00 | 8 × 10−2 | 42.00 | 1 × 10−4 | 18.00 | 8 × 10−4 | 4.00 | 5 × 10−1 |
| Tumour 2 | 8 × 10−3 | 1 × 10−3 | Not detected | 8 × 10−2 | 5 × 10−4 | 7 × 10−1 | Not detected | 6 × 10−2 | 3 × 10−3 |