Table 2

Comparison of screening methods

CIE-IHCIE-ComELISA-1ELISA-2WB-Com
Values are the number of positives detected in each category in sera from two groups without connective tissue disorders.
Both ELISA systems contained six antigens: Ro, La, Sm, U1-RNP, Scl70, and Jo-1.
Positive samples were subsequently negative using CIE and specific ELISAs for the individual antigens.
CIE-Com, countercurrent immunoelectrophoresis (commercial); CIE-IH, countercurrent immunoelectrophoresis (in house); ELISA, enzyme linked immunosorbent assay; RNP, ribonuclear protein; WB-Com, western blotting (commercial).
Normal blood donors (n = 100)21111Not tested
Normal pregnancy (n = 115)10017 (n = 60)