CIE-IH | CIE-Com | ELISA-1 | ELISA-2 | WB-Com | |
---|---|---|---|---|---|
Values are the number of positives detected in each category in sera from two groups without connective tissue disorders. | |||||
Both ELISA systems contained six antigens: Ro, La, Sm, U1-RNP, Scl70, and Jo-1. | |||||
Positive samples were subsequently negative using CIE and specific ELISAs for the individual antigens. | |||||
CIE-Com, countercurrent immunoelectrophoresis (commercial); CIE-IH, countercurrent immunoelectrophoresis (in house); ELISA, enzyme linked immunosorbent assay; RNP, ribonuclear protein; WB-Com, western blotting (commercial). | |||||
Normal blood donors (n = 100) | 2 | 1 | 11 | 1 | Not tested |
Normal pregnancy (n = 115) | 1 | 0 | 0 | 1 | 7 (n = 60) |