Primers used to amplify and identify markers in the cag pathogenicity island of Helicobacter pylori
| Region amplified | Primer designation | Primer sequence (5′ → 3′) | Amplicon size |
|---|---|---|---|
| ES, empty site; f, forward primer; r, reverse primer. | |||
| cagA | F1 | GATAACAGGCAAGCTTTTGAGG | 349 bp |
| B1 | CTGCAAAAGATTGTTTGGCAGA | ||
| cagA | D008 | TAATGCTAAATTAGACAACTTGAGCGA | 298 bp |
| R008 | TAGAATAATCAACAAACATCACGCCAT | ||
| cagD/E | picf | GAGCTTTTGCTTGTCTTTGC | 2091 bp |
| picr | CAAACAACGCTGCTTTCTAT | ||
| virD4 | 524f | TTCACCTCTAGTTTTAGCGG | 1993 bp |
| 524r | AACCTTTGGCTTTTGGTTGC | ||
| ES | ESf | ACATTTTGGCTAAATAAACGCTG | 360 bp |
| ESr | TCATGCGAGCGGCGATGTG | ||