Table 1

MPO-ANCA and PR3-ANCA ELISA, ANCA Combi-kit™ ELISA, and ANCA IIF results

IIF staining pattern (titre) on ethanol fixed neutrophils using polyclonal FITC conjugated antihuman IgG
SeraMPO-ANCA and PR3-ANCA IgG ELISA (U/ml)ANCA Combi-kit IgG ELISA (OD ratio)Inova DiagnosticsMBLIn house cytospin
ORGenTec MPO-ANCA and PR3-ANCA IgG ELISA: positive, >5 U/ml; negative, ≤5 U/ml.
ORGenTec ANCA Combi-kit® IgG ELISA OD ratio: positive, ≥1; negative, <1 (only positive results shown).
IIF staining pattern: C, classic granular cytoplasmic IIF staining with central/interlobular accentuation. P, perinuclear.
Sera 1–6 were from patients with biopsy confirmed microscopic polyangiitis.
Serum 7 was from a patient with biopsy confirmed Wegener's granulomatosis.
ANCA, antineutrophil cytoplasmic antibody; ELISA, enzyme linked immunosorbent assay; FITC, fluorescein isothiocyanate; IIF, indirect immunofluorescence; MPO, myeloperoxidase; OD, optical density; PR3, proteinase 3.
1MPO positive (58)MPO (6.4)P (1/160)P (1/160)P (1/160)
PR3 negativeAtypical cytoplasmic (1/160)
2MPO positive (61)MPO (2.9)P (1/160)P (1/160)P (1/160)
PR3 negativeAtypical cytoplasmic (1/10)
3MPO positive (8)MPO (1.6)P (1/40)P (1/160)P (1/160)
PR3 negativeAtypical cytoplasmic (1/40)
4MPO positive (6)MPO (1.3)P (1/10)P (1/40)P (1/40)
PR3 negativeAtypical cytoplasmic (1/40)
5MPO positive (>100)MPO (9.1)P (1/160)P (1/640)P (1/160)
PR3 negativeLysozyme (1.7)Atypical cytoplasmic (1/160)
6MPO positive (36)MPO (5.2)P (1/160)P (1/160)P (1/160)
PR3 negativeLactoferrin (1.1)Atypical cytoplasmic (1/10)
7MPO negativePR3 (6.44)C (1/40)C (1/40)C (1/40)
PR3 positive (53)