Species specific TaqMan™ primer and probe sequences and fluorescent labels
| Species | 5′ to 3′ primer and probe sequences and probe label | |
|---|---|---|
| Probe labels are highlighted in bold. | ||
| F, forward primer; R, reverse primer; P probe (phosphorylated at the 3′ end). | ||
| Multiplex A | ||
| Candida albicans | F | GGG TTT GCT TGA AAG ACG GTA |
| R | TTG AAG ATA TAC GTG GTG GAC GTT A | |
| P | FAM-ACC TAA GCC ATT GTC AAA GCG ATC CCG | |
| C kefyr | F | GGC TGC GTG TCG AGT CTA TG |
| R | TGA CCC AAG CTT ACC ACG AAT | |
| P | TET-ACT CTT GCA CAT CTA CGT CTT AGG TTT GCG C | |
| C glabrata | F | TTT CTC CTG CCT GCG CTT AA |
| R | ACG CAC ACT CCC AGG TCT TT | |
| P | VIC-AGA ACA CCC ACC AAC CGC GCA | |
| Multiplex B | ||
| C parapsilosis | F | GGG TTT GGT GTT GAG CGA TAC |
| R | GGA GTT TGT ACC AAT GAG TGG AAA | |
| P | FAM-CTC CGC CTT TCT TTC AAG CAA ACC CAG | |
| C tropicalis | F | CGT GGT AAC TTA TTT TAA GCG |
| R | GCT TAA GTT CAG CGG GTA GTC CTA | |
| P | TET-TGG CCA CCA TTT ATT TCA TAA CTT TGA CC | |
| C krusei | F | GCT GCG ACT CGC CTG AA |
| R | TTG TCT CGC AAC ACT CGC TCT | |
| P | VIC-CTA GTT CGC TCG GCC AGC TTC GCT | |