Calretinin | P | Zymed (San Francisco, USA) | 1/50 | Overnight/4° C | Hotplate | Cytoplasm |
CEA | M | Dako (Ely, UK) | 1/20 | 30–60 min/RT | Microwave | Cytoplasm |
Cytokeratin 5/6 | M | Dako | 1/50 | 30–60 min/RT | Microwave | Pericellular and cytoplasm |
BerEP4 | M | Dako | 1/50 | 30–60 min/RT | Pronase | Membrane |
E-cadherin | M | Zymed | 1/25 | Overnight/4° C | Hotplate | Membrane |
HBME-1 | M | Dako | 1/100 | 30–60 min/RT | No pretreatment | Membrane and cytoplasm |
LeuMI (CD15) | M | Dako | 1/40 | Overnight/4° C | Microwave | Cytoplasm |
N-cadherin | M | Zymed | 1/25 | Overnight/4° C | Hotplate | Cytoplasm |
SP-A | P | Chemicon (Harrow, UK) | 1/50 | Overnight/4° C | Hotplate | Membrane and cytoplasm |
Thrombomodulin | M | Dako | 1/100 | Overnight/4° C | No pretreatment | Membrane |
TTF-1 | M | Lab Vision (Newmarket, UK) | 1/50 | 30–60 min/RT | Microwave | Nucleus |