Table 1

Immunofluorescence patterns seen with four sera tested in 11 different laboratories

Laboratory	PMN	Serum dilution	Conjugate dilution	X	Serum A	Serum B	Serum C	Serum D
Neutrophil preparations and substrates from the same manufacturer have the same suffix; fluorescence is described as 0 to 3+; i0–3+, interlobular or central accentuation of cytoplasmic fluorescence; g0–3+, granularity of cytoplasmic fluorescence.
ANA, antinuclear antibody; C1–C6, conjugate preparations; H&L, antiserum against both heavy and light chains; P1–P3, neutrophil preparations; PMN, neutrophil preparations; X, magnification at which neutrophils were examined.
GRI	P1	1/40	C4 IgG (H&L), prediluted	400	Cytoplasmic 3+, i2+, g3+	Cytoplasmic 2+, i+, g−	Perinuclear with nuclear extension 3+, cytoplasmic 2+	Perinuclear rim only 3+
ARM	P1	1/20	C5 IgG F(ab`)₂, 1/100	400	Cytoplasmic 3+, i2+, g3+	Cytoplasmic 2+, i+, g+	Perinuclear with nuclear extension 3+, cytoplasmic 2+	Perinuclear rim only 3+
ALF	P1	1/40	C1 IgG prediluted	600	Cytoplasmic 3+, i +, g+	Not done	Perinuclear with nuclear extension 3+, cytoplasmic 2+	Perinuclear rim only 2+
STG	P1	1/40	C1 IgG prediluted	250	Cytoplasmic 2+, i2+, g2+*	Cytoplasmic 2+, i2+, g+	Perinuclear with nuclear extension 3+	Perinuclear rim only 3+
SOC	P2	1/20	C2 IgG, IgA, IgM, prediluted	400	Cytoplasmic 2+, i+, g+	Cytoplasmic 2+, flat atypical, i−, g−	Perinuclear with ? nuclear extension 3+	Perinuclear rim only 2+
RMH	P2	1/40	C6 Ig F(ab`)₂, 1/200	400	Cytoplasmic 2+, i2+, g+	Cytoplasmic 2+, i+, g−	Perinuclear with nuclear extension 3+, ? cytoplasmic extension 1+	Perinuclear rim only 3+
IMM	P3	1/20	C3 IgG (H&L) prediluted	400	Small cytoplasm, cytoplasmic   2+, i2+, g+	Cytoplasmic 2+, small cytoplasm, i+, g−	Perinuclear with nuclear extension 3+	? Perinuclear rim only 3+; almost cytoplasmic
FMC	P3	1/40	C3 IgG (H&L) prediluted	600	Small cytoplasm, cytoplasmic 2+, i2+, g+	Cytoplasmic 2+, small cytoplasm, i+, g−	Perinuclear with nuclear extension 3+	? Perinuclear rim only 3+, ?cytoplasmic extension
RBH	In house	1/10	C6 Ig (H&L), F(ab`)₂ 1/200	400	Cytoplasmic 3+, i+, g+	Cytoplasmic 2+, no outer margin, i +, g−	Perinuclear with nuclear extension 3+	Perinuclear rim only 3+
STV	In house	1/40	C5 IgG F(ab`)₂, 1/1000	400	Cytoplasmic 3+, i2+, g+	Cytoplasmic 2+, i+, g+	Perinuclear with nuclear extension 3+	Cytoplasmic 3+ (i 2+, g 2+)
RPA	In house	1/20	C1 IgG prediluted	400	Cytoplasmic 3+, i +, g−	Cytoplasmic 2+, i+, g+	Perinuclear with nuclear extension 3+	Perinuclear rim only 3+