Manufacturer's cut off point | ROC plot analysis | |||||||
---|---|---|---|---|---|---|---|---|
Assay type/Manufacturer | Cut off | CD (sensitivity) | Non-CD controls (specificity) | IBD controls | Threshold | CD (sensitivity) | Non-CD controls (specificity) | IBD controls |
Results greater than or equal to the cut off/threshold are considered positive. Specificity was calculated using only the non-CD controls (see text). | ||||||||
AU, arbitrary units; CD, coeliac disease; ELISA, enzyme linked immunosorbent assay; IBD, inflammatory bowel disease; IgA EMA, IgA anti-endomysial antibody; IgA tTG, IgA anti-tissue transglutaminase antibody; IIF, indirect immunofluorescence; NA, not applicable; tTG, tissue transglutaminase; ROC, receiver operating characteristic. | ||||||||
IgA EMA IIF/The Binding Site | 1/4 | 49/49 (100%) | 0/34 (100%) | 0/30 | NA | NA | NA | NA |
Human tTG based ELISA/Aesku.Lab | 15 U/ml | 35/49 (71%) | 0/34 (100%) | 0/30 | 4 U/ml | 47/49 (96%) | 0/34 (100%) | 1/30 |
Human tTG based ELISA/The Binding Site | 4 U/ml | 48/49 (98%) | 3/34 (91%) | 1/30 | 6 U/ml | 47/49 (96%) | 1/34 (97%) | 0/30 |
Human tTG based ELISA/Eurospital | 7 AU | 47/49 (96%) | 4/34 (88%) | 0/30 | 9 AU | 47/49 (96%) | 1/34 (97%) | 0/30 |
Human tTG based ELISA/QUANTA Lite | 20 U/ml | 48/49 (98%) | 0/34 (100%) | 0/30 | 16 U/ml | 48/49 (98%) | 0/34 (100%) | 0/30 |
Human tTG based ELISA/Orgentec | 10 U/ml | 49/49 (100%) | 5/34 (85%) | 1/30 | 11 U/ml | 48/49 (98%) | 2/34 (94%) | 0/30 |
Human tTG based ELISA/Varelisa | 5 U/ml | 49/49 (100%) | 0/34 (100%) | 0/30 | 4 U/ml | 49/49 (100%) | 0/34 (100%) | 0/30 |