Antibody/antigen | Dilution | Source | Pretreatment |
---|---|---|---|
HsMCM2 | 1/300 | Transduction Laboratories (Newington, New Hampshire, USA) | 2×5 minutes in microwave |
Cyclin A | 1/50 | Novocastra (Newcastle upon Tyne, UK) | 2×5 minutes in microwave |
Cyclin D1 | 1/200 | Oncogene Research (Manhasset, New York, USA) | 2×5 minutes in microwave |
Cyclin D3 | 1/25 | Dako (Carpenteria, California, USA) | 4×5 minutes in microwave |
Cyclin E | 1/100 | Santa Cruz Biotechnology (Santa Cruz, California, USA) | 4×5 minutes in microwave |
Ki-67 | 1/25 | Dako | 5×5 minutes in microwave |
PCNA | 1/500 | Novocastra | 2×5 minutes in microwave |