Summary of genetic analysis
| Case | Homogeneous LOH | Heterogeneous LOH | Genetic pattern | FAL |
|---|---|---|---|---|
| Microsatellite markers listed showed LOH for each case. | ||||
| α, β, allelic losses of the opposite chromosomal arms; complex, single clonal process cannot be confirmed because of the complexity of the LOH pattern; divergence, single clonal process with subsequent genetic divergence; homogeneous, all the foci show the same genetic alteration; progression, linear genetic progression of a clone. | ||||
| FAL, fractional allelic loss; LOH, loss of heterozygosity. | ||||
| HP1 | 3p (D3S1293), 5q (D5S644), 9p (D9S1748, D9S1752), 17p (CHRNB1), 18q (D18S474, D18S487, D18S55) | 13q (D13S171, D13S154) | Progression | 0.75 |
| HP2 | 3p (D3S1293), 5q (D5S644), 9p (D9S1748, D9S1752) | 13q (α, β) (D13S171, D13S263, D13S154) | Divergence | 0.57 |
| HP4 | 3p (D3S1234), 11q (Int2), 13q (D13S171), 18q (D18S474, D18S55) | Homogeneous | 0.67 | |
| HP5 | 13q (D13S260, D13S263, D13S168, D13S166, D13S154), 16q (D16S261) | Homogeneous | 0.40 | |
| HP6 | 3p (D3S1234, D3S1293), 5q (D5S647, D5S644), 8p (D8S261, D8S133), 13q (D13S260, D13S171, D13S168, D13S166), 16q (D16S261), 17p (TP53, D17S786) | Homogeneous | 0.67 | |
| HP7 | 3p (D3S1234, D3S1293), 16q (D16S265, D16S261) | 9p (D9S1748, D9S1752), 17p (TP53, CHRNB1) | Progression | 0.50 |
| HP8 | 13q (D13S154), 17p (TP53, D17S786, CHRNB1) | 8p (D8S133) | Progression | 1.00 |
| HP9 | 5q (D5S647, D5S644), 8p (D8S261, D8S133), 17p (TP53, CHRNB1), 18q (D18S474, D18S55) | 9p (D9S1748), 11q (Int 2), 13q (D13S260, D13S171, D13168, D13S166) | Divergence | 1.00 |
| HP12 | 3p (D3S1293), 5q (D5S644), 11q (D11S29), 16q (D16S265, D16S261), 17p (TP53, CHRNB1), 18q (D18S474) | 13q (D13S168, D13S260) | Progression | 0.88 |
| HP14 | 3p (D3S1234, D3S1293), 17p (D17S786, CHRNB1) | 5q (D5S644), 9p (D9S1748), 16q (D16S265) | Divergence | 0.65 |
| HP15 | 3p (D3S1293), 5q (D5S647, D5S644), 8p (D8S133), 9p (D9S1748, D9S1752), 11q (Int 2) | 13q (D13S171, D13S166, D13S154) | Progression | 0.86 |
| HP23 | 9p (D9S1748, D9S1752), 11q (Int2), 16q (D16S265), 17p, (CHRNB1, TP53), 18q (D18S487) | 13q (D13S171, D13S154) | Progression | 0.86 |
| HP24 | 3p (D3S1234), 5q (D5S647, D5S644), 8p (D8S133), 13q (D13S171, D13S166), 16q (D16S265) | Complex | 1.00 | |
| HP26 | 3p (D3S1234), 5q (D5S647), 13q (D13S171, D13S166), 16q (D16S265, D16S261) (only in invasive foci) | 3p (D3S1234), 5q (D5S647), 13q (D13S171, D13S166), 16q (D16S265, D16S261), 18q (D18S474, D18S55) (mucosal foci) | Complex (mucosa) Homogeneous (invasion) | 1.00 |
| HP27 | 3p (D3S1234) | 17p (α, β) ( D17S786, CHRNB1) | Divergence | 0.25 |
| HP36 | 13q (D13S171, D13S168, D13S166, D13S154), 17p (TP53) | Homogeneous | 0.50 | |
| HP38 | 5q (D5S644), 11q (D11S29, Int 2), 13q (D13S260, D13S171, D13S168), 17p (TP53) | Homogeneous | 0.67 | |
| HP40 | 3p (D3S1293), 5q (D5S647), 8p (D8S261, D8S133), 17p (CHRNB1) | 11q (Int 2, D11S29), 13q (D13S171, D13S168, D13S166, D13S154) | Divergence | 0.88 |
| HP41 | 3p (D3S1293), 5q (D5S647, D5S644), 9p (D9S1749, D9S1748), 18q (D18S474, D18S487) | Homogeneous | 0.57 | |