Results of the RQ-PCR assays using an MGB probe and a non-MGB probe in 18 patients with ALL
| Patient | Normalised values of PCR target detected by MGB probe* (those by non-MGB probe) | ||||
|---|---|---|---|---|---|
| Initial diagnosis | 15 days | 1 month | 3 months | 4 months | |
| ALL, acute lymphoblastic leukaemia; blank, not determined; MGB, minor groove binder; RQ-PCR, real time quantitative polymerase chain reaction; –, not detected. | |||||
| *The normalised values of targets were expressed as the ratio of the target copy number for each 105 cell equivalent from the integrated standard curves of human β actin, as described previously.4 | |||||
| 1 | 42700 (38000) | 0.34 (–) | – (–) | – (–) | 1.36 (0.74) |
| 2 | 17500 (11700) | 17.6 (15.4) | – (–) | – (–) | |
| 3 | 38200 (29400) | 14060 (16500) | 1280 (687) | – (–) | – (–) |
| 4 | 27100 (30300) | 33400 (34800) | – (–) | – (–) | |
| 5 | 15300 (9050) | 336 (425) | – (–) | ||
| 6 | 8700 (10700) | 870 (440) | |||
| 7 | 3160 (320) | 35.0 (57.0) | |||
| 8 | 7700 (4100) | 1.42 (0.73) | |||
| 9 | 69500 (68000) | 8550 (8400) | 5.84 (9.10) | ||
| 10 | 3350 (4540) | 210 (180) | |||
| 11 | 29000 (32800) | – (–) | – (–) | ||
| 12 | 19800 (20800) | 10.5 (7.6) | – (–) | ||
| 13 | 4840 (4290) | – (–) | – (–) | – (–) | |
| 14 | 2330 (2480) | – (–) | – (–) | ||
| 15 | 770 (535) | – (–) | – (–) | – (–) | |
| 16 | 23000 (18300) | – (–) | – (–) | ||
| 17 | 1250 (1430) | 6200 (530) | 350 (340) | ||
| 18 | 12830 (11250) | 17.4 (12.4) | – (–) | 4.9 (0.74) | |