Primers for PCR amplification of HHV8 K1, gBN, gBC, and gHM genes
| Gene | PCR round | Primer | Nucleotide position32 |
|---|---|---|---|
| The primers used were those used by Meng et al.10 | |||
| HHV8, human herpesvirus 8; PCR, polymerase chain reaction. | |||
| K1 | 1st | 5′-GGCCCTTGTGTAAACCTGT-3′ | 51–69 |
| 5′-AGTATCCGACCTCATAAAATG-3′ | 1081–1061 | ||
| 2nd | 5′-GACCTTGTTGGACATCCTGTA-3′ | 76–96 | |
| 5′-ACTGGTTGCGTATAGTCTTCC-3′ | 961–941 | ||
| gBN | 1st | 5′-GACCTGTACGCCCTTCTGTAC-3′ | 8653–8673 |
| 5′-ATAGAGTGGCACGGGTCTC-3′ | 9136–9118 | ||
| 2nd | 5′-GCCACCCTGGGGACTGTCAT-3′ | 8720–8739 | |
| 5′-TTGGTGATGGCGGACTCTGTC-3′ | 9102–9082 | ||
| gBC | 1st | 5′-CCTGGGTGGCATCGGAAAAAC-3′ | 10795–10815 |
| 5′-GCGTGGGTTGCCTCACAGTGT-3′ | 10432–11413 | ||
| 2nd | 5′-ATTGGTTACCGGATTCATAAA-3′ | 10858–10878 | |
| 5′-GGGTCGATAAATGGATTGA-3′ | 11391–11373 | ||
| gHM | 1st | 5′-GCGCTCTATGGAGTGGTGTC-3′ | 37689–37708 |
| 5′-CTAGAAAGCAGGGGGAGAATG-3′ | 38624–38605 | ||
| 2nd | 5′-GACGGCGTCCCATCTTCTGTT-3′ | 37760–37781 | |
| 5′-GGGCAGCTGTCGGTGAGG-3′ | 38473–38456 | ||