Table 1

 Details of the antibodies, dilutions, and antigen retrieval methods used

Primary antibodyDilution/incubation timeAntigen retrievalSpecificitySource
*At room temperature; †slides are placed in antigen unmasking buffer pH 6.0 (TAR001; YLEM) then microwaved (3×4 minutes); ‡predigestion with protease type XXIV for 20 minutes at 37°C.
HIF1α, hypoxia inducible factor-1α; LDH5, lactate dehydrogenase-5; TP, thymidine phosphorylase; VEGF, vascular endothelial growth factor.
Ki-671/75 (75 min*)MW†MIB1YLEM, Avezzano, Italy
P-GF.44C1/4 (75 min*)MW†TPOxford University, Oxford, UK
VG11/4 (75 min*)MW†VEGFOxford University
JC70 (CD31)1/20 (30 min*)Protease‡EndotheliumDako, Glostrup, Denmark
ESEE 1221/20 (overnight)MW†HIF1αOxford University
90021/100 (75 min*)MW†LDH5Abcam, Cambridge, UK