HIF1α and LDH5 grading system based on the intensity and extent of cytoplasmic and nuclear staining
| HIF1α/LDH5 grading system | Score |
|---|---|
| HIF1α, hypoxia inducible factor-1α; LDH5, lactate dehydrogenase-5. | |
| Complete absence of reactivity | Negative (low) |
| Weak cytoplasmic reactivity (regardless of extent) | Low |
| Strong cytoplasmic reactivity in less than 50% of tumour cells | Low |
| Nuclear expression in sporadic tumour cells (<10% of cells) | Low |
| Strong cytoplasmic expression in more than 50% of tumour cells | High |
| Nuclear expression in more than 10% of tumour cells | High |