Table 2

 HIF1α and LDH5 grading system based on the intensity and extent of cytoplasmic and nuclear staining

HIF1α/LDH5 grading systemScore
HIF1α, hypoxia inducible factor-1α; LDH5, lactate dehydrogenase-5.
Complete absence of reactivityNegative (low)
Weak cytoplasmic reactivity (regardless of extent)Low
Strong cytoplasmic reactivity in less than 50% of tumour cellsLow
Nuclear expression in sporadic tumour cells (<10% of cells)Low
Strong cytoplasmic expression in more than 50% of tumour cellsHigh
Nuclear expression in more than 10% of tumour cellsHigh