Immunohistochemical results (reactivity range, median value, and grouping of cases)
| Invading tumour front | Range | Median | High | Low |
|---|---|---|---|---|
| cyt, cytoplasmic staining; HIF1α, hypoxia inducible factor-1α; LDH5, lactate dehydrogenase-5; nuc, nuclear staining; TP, thymidine phosphorylase; VEGF, vascular endothelial growth factor. | ||||
| MIB1 index in fibroblasts | 0–20% | 2% | 72 | 78 |
| MIB1 index in cancer cells | 2–90% | 40% | 64 | 86 |
| TP expressing fibroblasts | 0–100% | 50% | 80 | 70 |
| TP expressing cancer cells | 0–100% | 0% | 8 | 142 |
| VEGF expressing cancer cells | 0–90% | 10% | 44 | 106 |
| Vascular density | 15–110 | 42 | 74 | 76 |
| HIF1α expressing cancer cells nuc/cyt (grading scale) | 58 | 92 | ||
| HIF1α nuc | 0–30% | 0% | ||
| HIF1α cyt | 0–100% | 5% | ||
| LDH5 expressing cancer cells nuc/cyt (grading scale) | 103 | 47 | ||
| LDH5 nuc | 0–70% | 0% | ||
| LDH5 cyt | 0–100% | 30% | ||