Primers and probes used for the detection of human papillomavirus (HPV) DNA by the polymerase chain reaction
| Primer/Probe | Nucleotide position | Product (bp) | |
|---|---|---|---|
| The primers and probes were those used by McNicol et al (1994)29 and Walboomers et al (1999).30 | |||
| HPV-6 E6 | |||
| Sense | 5′-GCTGGATATGCAACAACAGTTG-3′ | 348–369 | 189 |
| Antisense | 5′-CATGCATGTTGTCCAGCAGTG-3′ | 516–536 | |
| Probe | 5′-GCTACCTGTGTCACAAACCG-3′ | 412–431 | |
| HPV-11 E6 | |||
| Sense | 5′-GCAGCGTGTGCCTGTTGCTTA -3′ | 285–305 | 230 |
| Antisense | 5′-AAGCAACGACCCTTCCACTGG-3′ | 494–514 | |
| Probe | 5′-CCTGTGTCACAAGCCGTTGTG-3′ | 416–436 | |
| HPV-16 E6 | |||
| Sense | 5′-GATGGGAATCCATATGCTGTA-3′ | 269–289 | 240 |
| Antisense | 5′-TCGACCGGTCCACCGACCCCT-3′ | 488–508 | |
| Probe | 5′-GCCACTGTGTCCTGAAGAAAAGC-3′ | 427–449 | |
| HPV-16 E7 | |||
| Sense | 5′-CCAGAGACAACTGATCTCTAC-3′ | 610–630 | 171 |
| Antisense | 5′-GTGTGTGCTTTGTACGCACAAC-3′ | 759–780 | |
| Probe | 5′-TAACCTTTTGTTGCAAGTGTGACTCTACGCTTCG-3′ | 725–758 | |
| HPV-18 E6 | |||
| Sense | 5′-CAGTATACCCCATGCTGCATGCC-3′ | 278–300 | 159 |
| Antisense | 5′-GGTTTCTGGCACCGCAGGCA-3′ | 417–436 | |
| Probe | 5′-CAGACTCTGTGTATGGAGACAC-3′ | 349–370 | |
| HPV-18 E7 | |||
| Sense | 5′-GAGCCGAACCACAACGTCAC-3′ | 747–766 | 152 |
| Antisense | 5′-GGATGCACACCACGGACACA-3′ | 879–898 | |
| Probe | 5′-TCCAGCAGCTGTTTCTGAACACCCTG-3′ | 846–871 | |
| HPV-31 E7 | |||
| Sense | 5′-GGGCTCATTTGGAATCGTGTG-3′ | 811–831 | 100 |
| Antisense | 5′-AACCATTGCATCCCGTCCCC-3′ | 910–891 | |
| Probe | 5′-TACCTGCTGGATCAGCCATTGTAGTTACAG-3′ | ||
| HPV-33 E7 | |||
| Sense | 5′-TGAGGATGAAGGCTTGGACC-3′ | 671–690 | 110 |
| Antisense | 5′-TGACACATAAACGAACTGTG-3′ | 780–761 | |
| Probe | 5′-TGTGACAACAGGTTACAATGTAGTAATCAG-3′ | ||
| HPV-39 E7 | |||
| Sense | 5′-CCAAAGCCCACCTTGCAGGA-3′ | 601–620 | 123 |
| Antisense | 5′-ATGGTCGGGTTCATCTATTTC-3′ | 723–703 | |
| Probe | 5′-TCCTAATTGCTCGTGACATACAAGGTCAAC-3′ | ||
| HPV-45 E7 | |||
| Sense | 5′-CCCGACGAGCCGAACCACAG -3′ | 741–760 | 101 |
| Antisense | 5′-TCTAAGGTCCTCTGCCGAGC-3′ | 841–822 | |
| Probe | 5′-AGCTCAATTCTGCCGTCACACTTACAACAT-3′ | ||
| HPV-52 E7 | |||
| Sense | 5′-GCAGAACAAGCCACAAGCAA-3′ | 691–710 | 105 |
| Antisense | 5′-TAGAGTACGAAGGTCCGTCG-3′ | 795–776 | |
| Probe | 5′-ATAGCCGTAGTGTGCTATCACAACTGTGAC-3′ | ||
| HPV-58 E7 | |||
| Sense | 5′- CGAGGATGAAATAGGCTTGG-3′ | 672–691 | 109 |
| Antisense | 5′-ACACAAACGAACCGTGGTGC-3′ | 780–761 | |
| Probe | 5′-TGTTGTTCAATGTTACATCATTAATCGACA-3′ | ||