Table 2

Sequence of primers and PCR conditions for RNA analysis

Gene	Primer sequence 5′–3′	Length of product	Ta	Cycles
EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RT-PCR, reverse transcription polymerase chain reaction; Ta, annealing temperature.
β Actin	Forward: ATC ACT GCC CTG GCA CCC A	77 bp	60	30
	Reverse: CCG ATC CAC ACG GAG TAC TTG
β Actin	Forward: 5TTG CCG ACA GGA TGC AGA A	100 bp	60	30
	Reverse: the same primer used for β-Act 77 bases
β Actin	Forward: TGG AGA AGA GCT ACG AGC TG	120 bp	58	40
	Reverse : GAA GGT AGT TTC GTG CAT CG
β Actin	Forward: CTG GAC TTC GAG CAA GAG AT	170 bp	58	40
	Reverse: the same primer used for β-Act 120 bases
β Actin	Forward: GAG AAG CTG TCC TAC GTC G-3′	200 bp	59	45
	Reverse: the same primer used for β-Act 120 bases
EGFR	Forward: GGC TCT GGA GGA AAA GAA AG	65 bp	55	40
	Reverse: TCA AAA GTG CCC AAC TGC TG
EGFR	Forward: AAC ATC CTC TGG AGG CTG A	95 bp	55	40
	Reverse: TCA AAA GTG CCC AAC TGC TG
GAPDH	Forward: CCC TCA ACG ACC ACT TTG TCA A	75 bp	58	30
	Reverse: GGT CCA CCA CCC TGT TGC T
GAPDH	Forward: TCC ACC TTT GAC GCT GGG	103 bp	58	30
	Reverse: GGT CCA CCA CCC TGT TGC T