Antibodies, pretreatments, and dilutions
| Antibody | Clone | Source | Dilution | Target cells |
|---|---|---|---|---|
| Suppliers: N-C, Novocastra Laboratories, Newcastle upon Tyne, UK; Dako, Glostrup, Denmark; Serotec, Oxford, UK. | ||||
| *Pretreatment: microwave oven, boiled in citric acid pH 6.0 for 20 minutes; †pretreatment: enzyme digestion, 0.5% trypsin at 37°C for 30 minutes. | ||||
| CK, cytokeratin. | ||||
| CD3 | PS1 | N-C | 1/200* | T cells |
| CD4 | 1F6 | N-C | 1/20* | T helper cells |
| CD8 | 4B11 | N-C | 1/25* | T suppressor cells |
| CD20 | L26 | Dako | 1/1000* | B cells |
| CD117 | Polyclonal | Dako | 1/300* | Mast cells |
| CD138 | B-B4 | Serotec | 1/75* | Plasma cells |
| CD163 | 10D6 | N-C | 1/100* | Macrophages |
| CK7 | OV-TL 12/30 | Dako | 1/1000† | Epithelial cells |
| CK20 | KS 20.8 | Dako | 1/125† | Epithelial cells |