Primers and probes used in real time PCR assays
| Oligonucleotide sequence (5′–3′) | |
|---|---|
| Primers and TaqMan probes used in gene expression assays were designed in Molecular Baecon software (BioRad); primers used in the gene dosage assay were designed in Primer3 software (http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi). | |
| eRF, eukaryotic release factor; F, forward primer; P, TaqMan probe; PCR, polymerase chain reaction; R, reverse primer. | |
| Gene expression | |
| eRF1 | F: TGCATCTAACATTAAGTCACGAGT |
| R: TCCACAGTATACAACCAGACCATT | |
| P: AACCGCCTTTCAGTCCTGGGAGCC | |
| eRF3/GSPT1 | F: CGCCAGGTGCTCCTAAGAAAG |
| R: CAAATACATTATTTGTCCTCCAATGGT | |
| P: ACTTGCCAGCATCTACGTGCCCAATG | |
| eRF3/GSPT2 | F: GAGTTCGTGCCGTCCTTCCT |
| R: CCCGACTCAGCCGCCCACCCT | |
| P: CCGTCCCATCCTTTTACCTTGAG | |
| Gene dosage | |
| eRF3/GSPT1 | F: ATAATGATGTACACGGTCACAGTT |
| R: TTCTTGAAGAGAGATTAGTATGATGAA | |
| β Actin | F: AACACTGGCTCGTGTGACAA |
| R: CAGACCTACTGTGCGCCTACT | |