Table1

 Immunohistochemical staining methods

ProteinAntigen retrievalPrimary antibody
All sections were paraffin wax embedded.
COX-2, cyclooxygenase 2; iNOS, inducible nitric oxide synthase; MW, microwave oven.
iNOS2×15 minutes at 98°C in 1mM EDTA; pH 8.0Mouse monoclonal at 1/100 dilution; BD-Transduction Laboratories, San Jose, California, USA; catalogue no. 610431
COX-2MW; 2×15 minutes at 98°C in 1mM EDTA; pH 8.0Mouse monoclonal at 1/50 dilution; BD-Transduction Laboratories; catalogue no. 610203
FasMW; 2×15 minutes at 98°C in 10mM citrate; pH 6.0Mouse monoclonal at 1/400 dilution; Upstate Biotechnology, Lake Placid, Massachusetts, USA; catalogue no. 05–201
Bcl-xlMW; 2×15 minutes at 98°C in 0.1M Tris/HCl; pH 9.0Mouse monoclonal at 1/100 dilution; Zymed Laboratories, South San Francisco, California, USA; cat. no. 33–6300
Active caspase 3MW (700 W); 1×8 minutes in 10mM citrate buffer; pH 6.0Rabbit polyclonal at 1/100 dilution; Cell Signaling Technology, Beverley, Massachusetts, USA; catalogue no. 9661S