Primer and probe sequences used in real time quantitative polymerase chain reaction
| Gene | Primer sequence (5′–3′) | Hybridisation probe sequence (5′–3′) |
|---|---|---|
| All probes were labelled at the 5′ end with FAM as reporter and at the 3′ end with TAMRA as quencher. | ||
| HSA, human serum albumin. | ||
| CCNA | ||
| Sense | AAGAAGCCAGCTGAATCTCAAA | AAAAGCCAGGGCATCTTCACGCTCTATT |
| Antisense | GGTCCAGGTAAACTAATGGCTGAA | |
| CCNB1 | ||
| Sense | CCCTGCTGCAACCTCCAA | CCCGGACTGAGGCCAAGAACAGC |
| Antisense | TGTTCACTGACTTTGTTACCAATGTC | |
| CCNC | ||
| Sense | GCCGGCTGGTGCTTTTTTA | TGCCATGGAACACAGCTTGCCCT |
| Antisense | TGGGAGCTCTGCCAAAAGTT | |
| CCND1 | ||
| Sense | CCGTCCATGCGGAAGATC | CCTCCAGCATCCAGGTGGCGA |
| Antisense | AACAAGTTGCAGGGAAGTCTTAAGA | |
| CCND3 | ||
| Sense | CTGTCTCTCCCCGCCAGTT | CACCCCCGACACGTATTGTCT CCC |
| Antisense | CTGATATCTCAAGCTTTCCTTTTCCT | |
| CCNE | ||
| Sense | CCCCGCTGCCTGTACTGA | TCAGTGCCGACTCTGCCACATGG |
| Antisense | AGCATGGAGTAAGAGACCTGGAA | |
| CCNH | ||
| Sense | TATCCTTACCACACTTTTTTTCCTTCT | TCTACCAGGTCGTCATCAGTCCATTCTTCCT |
| Antisense | AGAAATCAACTTCAAATGGTTAGAGAGA | |
| HSA | ||
| Sense | TGTTGCATGAGAAAACGCCA | AAGTGACAGAGTCACCAAAT GCTGCACAG |
| Antisense | GTCGCCTGTTCACCAAGGAT | |