Table 1

 Primers and annealing temperatures for amplification of c-kit exons

ExonForward primersReverse primersFragment sizeAnnealing temperature (°C)
*Touchdown polymerase chain reaction with annealing temperature decreasing by 0.5°C every cycle to a “touchdown” annealing temperature of 60°C.
9GTATGCCACATCCCAAGTGTCATGACTGATATGGTAGACA33456
11CCAGAGTGCTCTAATGACTGGGAAGCCACTGGAGTTCCTT27456
13GACATCAGTTTGCCAGTTGTTGTTTTGATAACCTGACAGAC21456
17GCAACACTATAGTATTAAAAAGCCTTTGCAGGACTGTCAAGCA371TD 68*