Exon | Forward primers | Reverse primers | Fragment size | Annealing temperature (°C) |
---|---|---|---|---|
*Touchdown polymerase chain reaction with annealing temperature decreasing by 0.5°C every cycle to a “touchdown” annealing temperature of 60°C. | ||||
9 | GTATGCCACATCCCAAGTGT | CATGACTGATATGGTAGACA | 334 | 56 |
11 | CCAGAGTGCTCTAATGACTG | GGAAGCCACTGGAGTTCCTT | 274 | 56 |
13 | GACATCAGTTTGCCAGTTGT | TGTTTTGATAACCTGACAGAC | 214 | 56 |
17 | GCAACACTATAGTATTAAAAAG | CCTTTGCAGGACTGTCAAGCA | 371 | TD 68* |