PCR reaction mixtures for 5 DNA repair gene SNPs
| Reagents | XPA (Ala23Gly) (12.5 μl) | XPD-23 (Lys751Gln) (12.5 μl) | XPG-15 (Asp1104His) (12.5 μl) | XRCC3 -7 (Thr241Met) (12.5 μl) | XRCC1-6 (Arg194Trp) (6.25 μl) |
|---|---|---|---|---|---|
| F, forward; R, reverse; PCR, polymerase chain reaction; SNP, single nucleotide polymorphism. | |||||
| DNTP | 1 | 1 | 1 | 1 | 1 |
| MgCl2 | 1.25 | 1.25 | 1.25 | 1.25 | 1.25 |
| 10× buffer | 1.25 | 1.25 | 1.25 | 1.25 | 1.25 |
| Primer (F) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Primer (R) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Taq | 0.2 | 0.125 | 0.1 | 0.1 | 0.1 |
| H20 | 1.55 | 1.625 | 1.65 | 1.65 | 3.4 |
| DNA | 6.25 | 6.25 | 6.25 | 6.25 | 5 |