Sequence of primers used for nested PCR amplification of MBR/JH and MCR/JH
| Chromosome regions | Primer sequence |
|---|---|
| IGH, immunoglobulin heavy chain gene; JH, J region; MBR, major breakpoint region; MCR, minor cluster region; PCR, polymerase chain reaction. | |
| IGH JH external | 5′ ACC TGA GGA GAC GGT GAC 3′ |
| IGH JH internal | 5′ ACC AGG GTC CCT TGG CCC CA 3′ |
| bcl2 MBR external | 5′ CAG CCT TGA AAC ATT GAT GG 3′ |
| bcl2 MBR internal | 5′ TAT GGT GGT TTG ACC TTT AG 3′ |
| bcl2 MCR external | 5′ CGT GCT GGT ACC ACT CCT G 3′ |
| bcl2 MCR internal | 5′ GGA CCT TCC TTG GTG TGT TG 3′ |