HPV16 upstream E2-B primers and probe used in real time polymerase chain reaction assay for integration
| Physical state | Name | Sequence (5′→3′) | Tm (°C) | Primer site | Amplimer length (bp) |
|---|---|---|---|---|---|
| The E2-B system is located upstream of the previous E2-A system15 at HPV16 genomic primer positions 3362–3390 and 3427–3443. | |||||
| Tm was determined using the “Primer Express™” program, version 2.0 (PE Applied Biosystems). | |||||
| bp, base pairs; FP, forward primer; PRO, probe; RP, reverse primer; Tm, melting temperature. | |||||
| EpisomalA15 | Probe:16E2-APRO | (BODIPYR6G)-CACCCCGCCGCGACCCATA-(DQ) | 70 | ||
| Primer1:16E2-AFP | AACGAAGTATCCTCTCCTGAAATTATTAG | 59 | 3362–3390 | ||
| Primer2:16E2-ARP | CCAAGGCGACGGCTTTG | 60 | 3427–3443 | 82 | |
| EpisomalB | Probe:16E2-BPRO | AAGACGTTAGCCTTGAAGTGTATTTAACTGCACC | 67 | ||
| Primer1:16E2-BFP | ATTACAAGCAATTGAACTGCAACTAACGTT | 59 | 2962–2991 | ||
| Primer2:16E2-BRP | GTATTGCATATGTCTCCATCAAACTG | 58 | 3113–3138 | 177 | |