Table 2

 Expected immunohistochemical and histochemical staining reactions in epithelioid malignant mesothelioma and adenocarcinoma

Marker/stainMesotheliomaAdenocarcinoma
+, positive; −, negative.
*Positive in a small proportion of non-pulmonary adenocarcinomas.
†Nuclear staining in pulmonary adenocarcinoma and thyroid carcinoma.
‡Positive in some renal, ovarian and endometrioid carcinomas.
§Positive in most renal and urothelial carcinomas.
CEA, Monoclonal carcinoembryonic antigen; TTF-1 thyroid transcription factor-1; WT1, Wilms tumour gene 1; PAS-D, periodic acid-Schiff-D.
Pan-cytokeratin++
Cytokeratin 5/6+−*
Calretinin+
CEA+
B72.3+
TTF-1+†
Ber-EP4+
D2-40+
WT1+−‡
Thrombomodulin+−§
MOC-31+
BG-8+
CD15 (Leu-M1)+
Periodic acid-Schiff without diastase+ or −+
Periodic acid-Schiff with diastase (PAS-D)+
Alcian blue++/−
Alcian blue with hyaluronidaseIf + without digestion, remains + after digestion