Table 1

 Antibodies used in the immunohistochemical evaluation of the breast lesions

AntibodySpecificityRetrievalDilutionIncubation timeControl (lymph node)Distribution of staining signals
CD68HistiocytesCitrate, pH = 6Ready to use30 min at 37°CSinusoidalCytoplasmic, granular, diffuse
CD3Pan T cellsTrypsin1:10030 min at 37°CParacorticalMembranous
CD20Pan B cellsCitrate1:200Overnight at 4°CFollicularMembranous
Granzyme BCytotoxic T cellsCitrate, pH = 61:10030 min at 37°CParacorticalCytoplasmic granular, diffuse