Antibodies used in study with sources, dilutions and methods of pretreatment
| Antibody | Source | Dilution | Pretreatment |
|---|---|---|---|
| AE1/3 | Dako (Ely, UK) | 1:100 | Protease 1 (4 min) |
| Epithelial membrane antigen | Dako | 1:10 | See materials and methods |
| Desmin | Dako | 1:100 | See materials and methods |
| α smooth muscle actin | Dako | 1:500 | See materials and methods |
| h-caldesmon | Dako | 1:50 | Pressure cooker 2 min, pH 7.8 buffer; protease 3 (2 min) |
| α inhibin | Dako | 1:200 | See materials and methods |
| Calretinin | Zymed (San Francisco, CA, USA) | 1:50 | See materials and methods |
| Melan A | Dako | 1:10 | See materials and methods |
| CD99 | Dako | 1:10 | None |
| Oestrogen receptor (clone sp1) | Labvision (Fremont, CA, USA) | 1:200 | See materials and methods |
| Progesterone receptor | Dako | 1:50 | See materials and methods |
| Androgen receptor | Novocastra (Newcastle, UK) | 1:100 | See materials and methods |
| Vimentin | Dako | 1:400 | See materials and methods |
| WT1 | Dako | 1:50 | See materials and methods |
| CD10 | Novocastra | 1:5 | See materials and methods |
| CD56 | Novocastra | 1:50 | See materials and methods |
| Chromogranin | Dako | 1:600 | See materials and methods |
| HMB45 | Dako | 1:100 | See materials and methods |