Primer and probes used for quantitative reverse transcriptase polymerase chain reaction
| Gene name | Primers and probes (5′–3′) | Amplicon size (bp) |
|---|---|---|
| F, forward; P, probe; R, reverse. | ||
| Sequences for the forward and reverse primers as well as the FAM-TAMRA fluorescently labelled P are shown with corresponding amplicon sizes. | ||
| STAT3 | F-gccagagagccaggagca | |
| R-tgaagctgacccaggtagcgctgc | 75 | |
| P-acacagataaacttggtcttcaggtatg | ||
| Cyclin D1 | F-ccctgacggccgagaag | |
| R-aggttccacttgagcttgttcac | 103 | |
| P-aggagctgctgcaaatggagctg | ||
| Survivin | F-ggcccagtgtttcttctgctt | |
| R-gcaaccggacgaatgcttt | 91 | |
| P-agccagatgacgaccccatagaggaaca | ||
| Bcl-xl | F-aatgaccacctagagccttgga | |
| R-aggagaacggcggctgggatacttttgt | 78 | |
| P-tgctgcattgttcccatagagt | ||
| Mcl-1 | F-gacaaaacgggactggctagtt | |
| R-ccttctaggtcctctacatggaaga | 80 | |
| P-acaaagaggctgggatgggtttgtgg | ||