| Primer set | Sequence |
| 1 | F: 5′-GGTTTTCTTTTCTCCTCCAACC-3′ |
| R: 5′-TGCAGGACTGTCAAGCAGAG-3′ | |
| 2 | F: 5′-GATTTTGGTCTAGCCAGCGT-3′ |
| R: 5′-TGCAGGACTGTCAAGCAGAG-3′ | |
| 3 | F: 5′-GATGCGGCCGCTGTGTCCTTGGGAGATGTCA-3′ |
| R: 5′-GATGGCGCGCCTTGGCAAGGAAATACAGCAC-3′ | |
| 4 | F: 5′-GATGCGGCCGCGGTTTTCTTTTCTCCTCCAACC-3′ |
| R: 5′-GATGGCGCGCCTGCAGGACTGTCAAGCAGAG-3′ | |
| 5* | F: 5′-GATTTTGGTCTAGCCAGAGTCATCAAGAATGATTC-3′ |
| R: 5′-GAATCATCTTGATGACTCTGGCTAGACCAAAATC-3′ |
*The bases in bold text and underlined indicate the changes from the wild-type necessary to construct the D816V mutation.
F, forward; R, reverse.