Primary antibody | Dilution (incubation time) | Antigen retrieval | Specificity | Source | Reference |
ESEE 122 | 1:20 (90 min*) | MW | HIF-1α | Oxford University † | 11 |
EP 190b | Neat (90 min*) | MW | HIF-2α | Oxford University | 11 |
VG1 | 1:4 (90 min*) | MW | VEGF | Oxford University | 12 |
1D5 | 1:100 (60 min*) | MW | ER | Immunon-Shandon‡ | 13 |
1A6 | 1:100 (60 min*) | MW | PgR | Immunon-Shandon | 13 |
ab9002 | 25 μg/ml (75 min) | MW | LDH-5 | Abcam§ | 14 |
DO-7 | 1:30 (60 min) | MW | p53 | Dako¶ | 15 |
MoAb124 | 1:80 (60 min) | MW | bcl-2 | Dako | 15 |
JC70 (CD31) | 1:50 (60 min*) | Protease XXIV | Endothelium | Dako | 11 |
*At room temperature.
†Oxford University, Oxford, UK.
‡Immunon-Shandon, Pittsburgh, Pennsylvania, USA.
§Abcam, Cambridge, UK.
¶Dako, Glostrup, Denmark.
MW, microwave heating; VEGF, vascular endothelial growth factor; ER, oestrogen receptor; HIF, hypoxia-inducible factor; LDH, lactate dehydrogenase.