| Patient no | No years sick | VP1 stain | RNA-later sp. | BGMK cell culture | |||||
| Normal cells | 5-idu, dex-treated cells | ||||||||
| EV RNA | Primary culture* | 1st pass† | |||||||
| 4 wk | 4 wk | 6 wk | 8 wk | 4 wk | 8 wk | ||||
| 1 | 5 | – | – | – | ++ | +++ | – | +++ | – |
| 2 | 4 | nd | nd | – | – | +++ | – | ++ | – |
| 3 | 6 | ++ | + | – | – | +++ | +++ | + | – |
| 4 | 10 | ++ | + | – | ++ | ++ | – | +++ | – |
| 5 | 12 | ++ | + | – | – | ++ | – | ++ | – |
| 6 | 4 | + | – | – | – | + | – | nd | – |
| 7 | 8 | – | nd | – | +++ | ++ | ++ | + | – |
| 8 | 6 | nd | – | – | – | + | – | – | – |
| 9 | 10 | + | +++ | – | + | ++ | ++ | ++ | – |
| 10 | 13 | + | – | – | + | nd | nd | nd | nd |
| 11 | 3 | ++ | ++ | – | – | nd | nd | nd | nd |
| 12 | 15 | ++ | ++ | – | – | nd | nd | nd | nd |
| 13 | 20 | – | – | – | – | nd | nd | nd | nd |
5-idu, 5-idoxyuridine; dex, dexamethasone; EV, enterovirus; VP1, viral capsid protein 1.
Three to four biopsies were obtained from each patient’s antrum and processed for VP1 staining, RNA assay and viral culture. VP1 staining was graded as described in methods. EV RNA was determined on the RNA-later specimens and culture lysates at periods indicated. The optical density reading of the RT-PCR ELISA was rated as +, if >1.0, <1.9; ++, if >2.0, <3.0; +++, if ⩾3.0; nd, not done.
*The tissue homogenates were inoculated onto untreated BGMK cells (normal) or 5-idu- and dex-treated BGMK cells; half of the tissue culture monolayers were scraped and lysed and EV RNA determined at the time period indicated.
†Lysates from the 6-week-old primary culture were passed to another set of uninfected, 5-idu- and dex-treated cells, and the presence of EV RNA was assayed at the time period indicated.