Table 1 Immunohistochemical detection system
Antibody, cloneDilutionSourcePretreatment
OSF-2, Periostin rabbit polyclonal1:1500Biovendor LaboratoryNone
1:500 on cell cultures
VEGFR-1 rabbit polyclonal1:25Novus BiologicalsWaterbath *
VEGFR-2 rabbit polyclonal1:50Novus BiologicalsWaterbath*
VEGF (clone 5C3.F8)1:50AbcamWaterbath*
HER-2 (c-erbB-2)HercepTestDakoCytomationWaterbath*
ER (clone 1D5)1:100DakoCytomationWaterbath*
PR (clone 636)1:100DakoCytomationWaterbath*
Ki-67 (clone MIB-1)1:100DakoCytomationWaterbath*
Vimentin1:100DakoCytomationWaterbath†

  • *Waterbath, at 98°C for 40 min in 0.01 M citrate buffer, pH 6.0.

  • †Waterbath, at 98°C for 20 min in 0.01 M citrate buffer, pH 6.0.