Table 2 Spectrophotometrical and PCR results for DNA obtained with different extraction methods
Commercial DNA isolationDNA Digestion
HPPTPNucliSENS easyMAGQIAamp DNA MiniProteinase K
Spectrophotometry†Mean A260/A280 ratio1.26 (0.07)1.29 (0.07)1.38 (0.19)1.18 (0.09)
Mean DNA (ng/µl)3.63 (2.41)6.27 (5.84)9.34 (11.62)261.16 (425.23)*
Range of DNA (ng/µl)0.00–7.950.00–18.800.00–27.4022.55–1162.90
Conventional PCRMean amplified DNA (ng/µl)68.19 (37.89)63.33 (30.48)94.11 (54.25)¶43.03 (38.71)
Range of DNA (ng/µl)32.19–126.650.00–101.0426.13–174.770.00–128.82

  • Values in parentheses are SD.

  • †Spectrophotometrical results in this study are not conclusive for DNA quality.

  • *DNA concentration was calculated from A260 and was statistically different between the proteinase K method and the other DNA isolation techniques (p<0.0001).

  • ¶Amplified DNA was significantly different between the QIAamp and the Proteinase K method (p = 0.043).